conotoxin – Pandemic Timeline https://pandemictimeline.com Chronological Sequence of Events Sun, 08 Oct 2023 01:30:02 +0000 en-US hourly 1 https://wordpress.org/?v=6.7.2 https://pandemictimeline.com/wp-content/uploads/2021/06/Covid-150x150.ico conotoxin – Pandemic Timeline https://pandemictimeline.com 32 32 Salk Institute and University of Utah file patent US5969096: Conotoxin peptides https://pandemictimeline.com/1998/06/salk-institute-and-university-of-utah-file-patent-5969096-conotoxin-peptides/ Fri, 26 Jun 1998 00:00:05 +0000 https://pandemictimeline.com/?p=11507 A paralytic peptide, ψ-conotoxin Piiie has been purified and characterized from Conus purpurascens venom. Electrophysiological studies indicate that the peptide inhibits the nicotinic acetylcholine receptor (nAChR). However, the peptide does not block the binding of α-bungarotoxin, a competitive nAChR antagonist. Thus, ψ-conotoxin Piiie appears to inhibit the receptor at a site other than the acetylcholine-binding site. As ascertained by…

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Conus purpurascens

A paralytic peptide, ψ-conotoxin Piiie has been purified and characterized from Conus purpurascens venom. Electrophysiological studies indicate that the peptide inhibits the nicotinic acetylcholine receptor (nAChR). However, the peptide does not block the binding of α-bungarotoxin, a competitive nAChR antagonist. Thus, ψ-conotoxin Piiie appears to inhibit the receptor at a site other than the acetylcholine-binding site. As ascertained by sequence analysis, mass spectrometry, and chemical synthesis, the peptide has the following covalent structure:  HOOCCLYGKCRRYOGCSSASCCQR* (O = 4-trans hydroxyproline; * indicates an amidated C-terminus). The disulfide connectivity of the toxin is unrelated to the α- or the αA-conotoxins, the Conus peptide families that are competitive inhibitors of the nAChR, but shows homology to the μ-conotoxins (which are Na+ channel blockers).

— Biochemistry article

Why is it important that the ψ-conotoxin not block the binding of the α-bungarotoxin?

Substantially pure conotoxin peptides are provided which inhibit synaptic transmissions at the neuromuscular junctions and which are useful both in vivo and in assays because they specifically target particular skeletal nAChRs to the exclusion of neuronal nAChRs. The peptides are of such length that they can be made by chemical synthesis, and the preferred peptides have formula: H-His-4Hyp-4Hyp-Cys-Cys-Leu-Tyr-Gly-Lys-Cys-Arg-Arg-Tyr-4Hyp-Gly-Cys-Ser-S er-Ala-Ser-Cys-Cys-Gln-Xaa.sub.24 -NH.sub.2 wherein Xaa.sub.24 is Arg or Gly.

⋮

This invention was made with Government support under Grant Nos. GM-48677, GM-22737 and AM-26741, awarded by the National Institutes of Health.  The Government has certain rights in this invention.

— United States Patent 5.969.096: Conotoxin peptides

Sources:

  • Patent
    October 19, 1999. Ki-Joon Shon, William R. Gray, John Dykert, Doju Yoshikami, Maren Watkins, David R. Hillyard, Jean E. F. Rivier, and Baldomero M. Olivera. Conotoxin peptides. 5969096, filed June 26, 1998, and issued October 19, 1999.
    https://patents.google.com/patent/US5969096,
    https://patents.justia.com/patent/5969096.
    Patent.
    Local copy.
  • Research Journal
    August 1, 1997. Ki-Joon Shon, Michelle Grilley, Richard Jacobsen, G. Edward Cartier, Chris Hopkins, William R. Gray, Maren Watkins, David R. Hillyard, Jean Rivier, Josep Torres, Doju Yoshikami, and Baldomero M. Olivera. “A Noncompetitive Peptide Inhibitor of the Nicotinic Acetylcholine Receptor from Conus Purpurascens Venom.” Biochemistry 36 (31): 9581–87.
    https://doi.org/10.1021/bi970235w.
    Research Journal.

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]]> “Dr. Ardis Was WRONG” https://pandemictimeline.com/2022/10/dr-ardis-was-wrong/ Fri, 28 Oct 2022 00:00:59 +0000 https://pandemictimeline.com/?p=11440 Dr. Jane Ruby:  So tell us.  What did you get wrong? Dr. Bryan Ardis:  Yeah, it’s very interesting.  You reached out to me.  I said, wow, I had no idea.  When we first started talking about it, when Watch the Water dropped with Stew Peters, I was absolutely convinced from the research studies out of China…

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Dr. Jane Ruby:  So tell us.  What did you get wrong?

Dr. Bryan Ardis:  Yeah, it’s very interesting.  You reached out to me.  I said, wow, I had no idea.  When we first started talking about it, when Watch the Water dropped with Stew Peters, I was absolutely convinced from the research studies out of China and in France in the early parts of the pandemic, in January and April of 2020, both countries, the geneticists and the scientists actually termed that the origin source for the spike proteins and for the antibodies attacking the actual illness in China, they said the origin source for the disease was from two snakes called the Chinese krait snake and the Chinese king cobra.

Dr. Ruby:  Right.

Dr. Ardis:  But I was wrong about that.  It wasn’t actually just two snakes.  Two months later, Italy scientists, when they analyzed the blood, urine, and feces of every COVID-positive patient in Italy, they found a combination of 36 different animal venoms inside of only the COVID-19 patients, and they found zero of these venoms inside of the negative PCR tested COVID patients.  They did not have COVID, those who were negative.  They not only found snake venoms, Jane Ruby.  They found 20 different snake venoms inside the blood, in the feces of only COVID-19 patients.

Dr. Ruby:  So let me have you articulate the overarching because I’m going to have you take us through a few different pictures and some incredible information.  The idea generally is that certain— First of all, certain populations were targeted, but we’re all targeted, ultimately.  But that this material, and we’ll talk about how it gets into your body in a minute.  It’s actually designed to have you make your own COVID-19, if certain pieces are in place.  Did I just get that?

Dr. Ardis:  Yeah.  One of the things that—  Very impressive you wrapped it up that way and summarized it that way.

Dr. Ruby:  Now, let’s unpack it.

Dr. Ardis:  Yes, so what they found was 36 different total animal venoms found inside of COVID-19 patients’ bodily fluids, blood and feces.  They also evaluated for urine but didn’t find it in the urine.  They found it in just the blood and in the feces.  Twenty different snake venoms—and this is the mystery I did not know when Watch the Water dropped—I did not know that there were also toxic deadly venoms synthetically made from … ocean snails and a starfish called the Crown of Thorns Starfish.

— LIVE @7PM: Worldwide Exclusive: Dr. Ardis Was WRONG

Dr. Ardis goes on to explain how these deadly toxins are made through the process of genetic engineering.  E. coli has proven to be an efficient microorganism for the manufacture of these synthetic venoms, as well as yeasts.  Once exposed to the synthetic venoms, certain of these microorganisms within the body can become makers of these venoms.  Those most at risk are diabetics and races predisposed to diabetes such as Native Americans, blacks, and Hispanics.

This might suggest a ketogenic diet as a preventative and treatment measure, though perhaps not for mere obesity reduction, but rather for improvement of blood sugar control as well as the reduction of microorganisms such as yeasts and E. coli which feast on excess glucose.

Toxin-like peptides, almost identical to toxic components of venoms from animals, like conotoxins, phospholipases, phosphodiesterases, zinc metal proteinases, and bradykinins, were identified in samples from COVID-19 patients, but not in control samples.

— Italian study

“ … this enzyme [similar to an enzyme in rattlesnake venom] is trying to kill the virus, but at a certain point it is released in such high amounts that things head in a really bad direction, destroying the patient’s cell membranes and thereby contributing to multiple organ failure and death.”

— Dr. Floyd (Ski) Chilton, quoted in Like Venom Coursing Through the Body

Dr. Ruby:  What is the PCR test picking up?

Dr. Ardis:  The whole world was angry medically that they were now being forced to put faith and trust in what was called a PCR test to diagnose a new novel respiratory virus.  We have covered that ad nauseum, that is not an accurate test to diagnose viral infections of any kind.

Dr. Ruby:  What is it good at finding, Dr. Ardis?

Dr. Ardis:  However, imagine my shock to find out that for over two decades, it’s been used solely in every snake venom research ever to identify genetic materials of snake venom present in medium.  It [the PCR test] identifies snake venom genetics.

— LIVE @7PM: Worldwide Exclusive: Dr. Ardis Was WRONG

I want you all to know that there are certain things we know that actually stop venom from hurting the human body:

  • nicotine
  • mucuna pruriens, which is an extract from the velvet bean plant
  • NAC
  • vitamin C
  • EDTA, which is by far my favorite right now, along with nicotine
  • and then also glutathione
  • and then copper and zinc, as I mentioned already.  Everyone needs to be supplementing with copper and zinc right now.  They’re attacking your copper and zinc reservoirs with these venoms.

— Dr. Bryan Ardis, at 57:03

Please, medical professionals around the world, consider looking at your COVID variant patients in the future as weaponized envenomated patients from all kinds of venoms, and then start addressing it.  How do you treat an envenomated person?  You already know how to do that.

— Dr. Bryan Ardis

This invention was made with Government support under Grant Nos. GM-48677, GM-22737 and AM-26741, awarded by the National Institutes of Health.  The Government has certain rights in this invention.

— United States Patent 5.969.096: Conotoxin peptides

Therefore, a synthetic version of ω-conotoxin M VII A has found application as an analgesic drug ziconotide (Prialt).

— Wikipedia: Conotoxin

Among serious side effects of ziconotide, as an example of potential side effects of conotoxin:

  • holding false beliefs that cannot be changed by fact

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